This detailed volume explores tools to study epigenetics in cancer and to manipulate the proteins involved, critical to our understanding of mechanisms in cancer initiation and progression. This book compiles protocols for detecting core epigenetic modifications including DNA methylation, histone modifications, highlighting chromatin immunoprecipitation (ChIP), CUT&RUN, and advanced microscopy for visualizing chromatin architecture in situ. Additionally, it features chromatin accessibility protocols, methods to detect long non-coding RNAs and microRNAs from challenging clinical samples, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Epigenetics: Methods and Protocols serves as a practical resource for researchers at all stages on their journey to unlock the secrets of cancer epigenomes, and ultimately, to improve patient outcomes.