This volume provides comprehensive methods used to perform super-resolution microscopy and its analysis. Each chapter is written by a leading expert in the field, covering fundamentals of super-resolution microscopy, single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, expansion microscopy, and others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Super-Resolution Microscopy: Methods and Protocols provides well-established protocols used by both academics and industrials.